The U-87 MG cell line was established by Ponten·J et al. and is derived from a malignant glioma; subcutaneous inoculation of U-87 MG cells in nude mice can result in tumor formation.

Cell Name: Human glioblastoma cell line

Cell Synonyms: U-87 MG; U-87MG; U-87 MG; U87 MG; U-87-MG; U87-MG; U87MG; U-87; U87; 87 MG; 87MG

Catalogue No.: TCH-C367

Species: Human

Tissue Origin: Brain

Disease Characteristic: Brain Astrocytoma Glioblastoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: MEM + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G367

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 36-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection