This cell line was isolated from the glioblastoma tissue of a patient.

Cell Name: Human glioblastoma cell line (STR-authenticated)

Cell Synonyms: U251; U-251 MG; U-251-MG; U-251_MG; U251-MG; U251MG;   U-251; U251n; U251N; 251 MG; 251MG

Catalogue No.: TCH-C366

Species: Human
Tissue Source: Neural Glia
Disease Characteristic: Glioma (Glioblastoma)
Morphology: Fibroblast-like
Growth Properties: Adherent growth
Culture Medium: DMEM-H + 10% FBS + 1% P/S
Supplied Medium No.: TCH-G366
Passage Ratio: 1:3–1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma