The cell line (originally named 2T) was established in 1964 by Ponten J and Saksela E from a moderately differentiated sarcoma of the tibia in a 15-year-old girl. No viruses were detected through co-cultivation with WI-38 or by complement fixation (CF) assays for anti-simian vacuolating virus SV40, respiratory syncytial virus RSV, or adenovirus. The cells express receptors for insulin-like growth factors I and II (IGF-I, IGF-II) and osteosarcoma-derived growth factor (ODGF).

Cell Name: Human osteosarcoma cell line (STR authenticated)

Cell Synonyms: U-2 OS; U2OS

Catalogue No.: TCH-C365

Species: Human
Tissue Origin: Bone
Disease Characteristic: Osteosarcoma
Morphology: Epithelial-like
Growth Characteristics: Adherent
Culture System: McCoy’s 5A + 10% FBS + 1% P/S
Matching Medium Code: TCH-G365
Passage Ratio: 1:3–1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma