The TT cell line was established from a fine-needle biopsy sample of a 77-year-old female patient with medullary thyroid carcinoma. The TT cells continuously produce high levels of calcitonin and CEA. At 24 hours and 72 hours after medium replacement, immunoreactive calcitonin concentrations detected in the culture medium were 3900 pg per million cells and 7700 pg per million cells, respectively. After 72 hours, the accumulated concentration of CEA exceeded 27 ng per million cells.

Cell Name: Human medullary thyroid carcinoma cell line

Cell Synonyms: TT; MTC-TT

Catalogue No.: TCH-C362
Species: Human
Tissue Origin: Thyroid
Disease Characteristic: Thyroid Cancer

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: Ham's F-12K + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G362

Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 48-96 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection