The cell line was established from the peripheral blood of a 1-year-old boy with acute monocytic leukemia. The cells are capable of phagocytosing latex particles and activated red blood cells, and neither the cell membrane nor the cytoplasm contains immunoglobulins. They express C3R and FcR; can be induced to differentiate toward the monocytic lineage by the phorbol ester TPA; and can serve as a transfection host.

Cell Name: Human acute monocytic leukemia cell line (STR authenticated)

Cell Synonyms: THP-1; THP1; THP 1; THPI; THP-1(ATCC); O-THP-1;   Tohoku Hospital Pediatrics-1

Catalogue No.: TCH-C361

Species: Human
Tissue Origin: Peripheral Blood
Disease Characteristics: Acute Monocytic Leukemia
Morphology: Monocyte-like
Growth Properties: Suspension Culture
Culture System: RPMI-1640 + 10% FBS + 0.05 mM β-mercaptoethanol + 1% P/S
Matching Medium Number: TCH-G361

Passage Ratio: 1:3-1:6, with medium change every 2-3 days

Doubling Time: 24-48 hours
Culture Conditions: Atmosphere: 95% Air + 5% CO₂, Temperature: 37°C
Freezing Conditions: 60% Basal Medium + 30% FBS + 10% DMSO, Storage in Liquid Nitrogen
Quality Control: Negative for bacteria, fungi, and mycoplasma