The TCCSUP lineage was isolated in 1974 from an anaplastic transitional cell carcinoma (TCC) of the bladder neck.

Cell Name: Human bladder transitional carcinoma cell line

Cell Synonyms: TCCSUP; TCCSuP; TCC-SUP; TCC Sup

Catalogue No.: TCH-C356

Species: Human

Tissue Origin: Bladder

Disease Characteristics:Bladder Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G356

Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection