The T84 is a transplantable human cancer cell strain established from a lung metastasis of a 72-year-old male patient with colon cancer. The tumor tissue was subcutaneously inoculated into BALB/c nude mice and continuously passaged. Throughout the transplantation process in nude mice, the cell line consistently maintained the original histological characteristics of colon cancer. The T84 was established after 23 passages in athymic mice.
T84 cells grow to confluence as a monolayer and exhibit tight junctions and desmosomes between contacting cells. They possess numerous receptors for peptide hormones and neurotransmitters that are involved in maintaining directional electrolyte transport. T84 cells display tight junctions and desmosomes between adjacent cells, and they are positive for keratin immunoperoxidase staining.
Culture Medium:DMEM/F12 + 10% FBS+ 1% P/S Matching Medium Catalog Number: TCH-G354 Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days Doubling Time:48-72 h Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201 Quality Control: Negative for bacteria, fungi, and mycoplasma detection