The T-47 cell line was isolated by Keydar I et al. from the pleural effusion of a 54-year-old female patient with invasive ductal carcinoma of the breast. This cell line is a differentiated epithelial subline of the T-47 cells. It has been reported to possess cytoplasmic junctions and receptors for 17β-estradiol and other steroid hormones, as well as for calcitonin. The cells are capable of forming colonies in agarose and express the WNT7B oncogene.

Cell Name: Human breast ductal carcinoma cell line

Cell Synonyms: T-47D; T-47-D; T47-D; T47D:A; T47D

Catalogue No.: TCH-C353
Species: Human
Tissue Origin: Breast
Disease Characteristic: Ductal Carcinoma
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G353
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 36-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO; stored in liquid nitrogen
Quality Control: Negative for bacteria, fungi, and mycoplasma