Product Name:Luciferase Reporter T24 Cell Line
HyCyte cell line:HyCyte T24-Luc
Catalog No.:TCH-C352L
Construction method:Lentivirus
Selection Marker:Puromycin
Parental:T24
Species:Human
Morphology:Epithelioid
Growth properties:Adherent
Culture Medium:McCoy's 5A Medium supplemented with 10 % FBS, 4.0ug/mL Puromycin,100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G352L).
Passage:1:2-1:4
Doubling time:24-48 h
Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.
Freezing media:McCoy's 5A + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).