The T24 cell line is derived from the metastatic adenocarcinoma of a patient and exhibits cytotoxicity against T24 and related cell lines. The population doubling time of T24 cells is 19 hours, and they contain the ras (H-ras) oncogene.

Cell Name: Human bladder carcinoma cell line (STR authenticated)
Cell Synonyms: T24
Catalogue No.: TCH-C352
Species: Human
Tissue Origin: Bladder
Disease Characteristics: Bladder transitional cell carcinoma

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: McCoy's 5A + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G352

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection