SW480 was derived from a primary rectal adenocarcinoma of a 51-year-old Caucasian male patient, while SW620 originated from a lymph node metastasis of the same patient one year later. These cells are negative for CSAp (colon-specific antigen) and rectal antigen 3, but positive for keratin. The p53 gene has a G→A mutation at codon 273 (resulting in an Arg→His substitution) and a C→T mutation at codon 309 (leading to a Pro→Ser substitution). The p53 protein expression level is elevated. The oncogenes c-myc, K-ras, H-ras, N-ras, myb, sis, and fos are expressed positively, whereas N-myc oncogene expression is not detected. The cells do not express Matrilysin (a metalloproteinase associated with tumor invasion). It has been reported that these cells express GM-CSF (granulocyte-macrophage colony-stimulating factor). The ras proto-oncogene is also present.

Cell Name: Human colorectal adenocarcinoma cell line (STR-authenticated)

Cell Synonyms: SW480; SW-480; SW 480; SW480E

Catalogue No.: TCH-C348

Species: Human
Tissue Origin: Colon
Disease Characteristics: Colon Adenocarcinoma
Morphology: Epithelial-like
Growth Properties: Adherent
Culture System: L-15 + 10% FBS + 1% P/S
Matching Medium No.: TCH-G348
Passage Ratio: 1:3–1:6, change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: Air, Temperature: 37°C
Cryopreservation Conditions: 60% Basal Medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma