The cell line was established in August 1971 from a 55-year-old Caucasian female patient with adrenocortical carcinoma. The patient was pathologically diagnosed with a Grade IV primary small cell carcinoma of the adrenal cortex. Electron microscopy revealed that the cells exhibited numerous spherical gap junctions (BGJ).

Cell Name: Human adrenal cortex adenocarcinoma cell line

Cell Synonyms: SW-13; SW 13; Scott and White No. 13

Catalogue No.: TCH-C347

Species: Human

Tissue Origin: Kidney

Disease Characteristics: Adrenocortical Small Cell Carcinoma

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: L-15 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G347

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: Air, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection