The SW 1353 cell line was originally isolated from a primary Grade II chondrosarcoma of the right humerus in a 72-year-old Caucasian female. The original culture medium was L-15 medium supplemented with cortisone and insulin, along with 10% FBS and 1% antibiotics. In January 1982, ATCC received one vial of SW 1353 cells that had been passaged to passage 12 and cryopreserved.

Cell Name: Human chondrosarcoma cell line

Cell Synonyms: SW-1353; SW 1353;SW 1353;SW1353

Catalogue No.: TCH-C342
Species: Human
Tissue Origin: Bone
Disease Characteristic: Chondrosarcoma
Morphology: Fibroblast-like
Growth Properties: Adherent
Culture Medium: L-15 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G342

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: Air , Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection