Immortalized human uroepithelial cell line(SV-HUC-1)
Cat.No. Size
Parameters
Detail
Cell Info
References
Cat.No.
TCH-C341
Size
1x10^6
Price
$800.00
The SV-HUC-1 cell line was established by transfecting normal ureteral tissue with the SV40 virus. Repeated testing using African green monkey kidney cell monolayers to detect the production of infectious SV40 has consistently yielded negative results; however, under stress conditions (such as exposure to chemical agents), the virus may become activated in SV-HUC-1 cells.
Cell Name: Immortalized human uroepithelial cell line
Cell Synonyms: SV-HUC-1; HUC-1; SV-HUC; SVHUC
Catalogue No.: TCH-C341 Species: Human Tissue Origin: Ureter
Disease Characteristics:Prostate Cancer Morphology: —
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days Doubling Time: 24-48 h Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201 Quality Control: Negative for bacteria, fungi, and mycoplasma detection
Subculture of SV-HUC-1 Cells
Aspirate and discard the used cell culture medium from the culture vessel.
Rinse the cells with PBS (approximately 2 mL solution per 10 cm² of culture surface area). Gently add the rinsing solution from the side of the container opposite to the adherent cell layer to avoid disturbing the cell layer. Swirl the container gently back and forth several times.
Aspirate and discard the rinsing solution from the culture vessel, then add 0.25% trypsin to the flask (the volume should be sufficient to cover the cell layer).
Gently swirl the container to ensure the reagent fully covers the cell layer. For a T25 culture flask, retain about 200 μL of the solution.
Place the culture vessel in the incubator and incubate for approximately 5 minutes. Observe cell detachment under a microscope.
When ≥90% of the cells have detached, tilt the culture vessel to allow the liquid on the cells to drain as quickly as possible. Add complete medium at twice the volume of the trypsin used, and pipette the cell layer surface several times to disperse the medium.
Transfer the cells to a sterile centrifuge tube and centrifuge at 200×g for 3-5 minutes.
Resuspend the cell pellet in the minimal volume of complete medium. Dilute the cell suspension at the recommended ratio, transfer an appropriate volume of the suspension to a new culture vessel, and return the cells to the incubator.