Product Name:Luciferase Reporter SK-OV-3 Cell Line
HyCyte cell line:HyCyte SK-OV-3-Luc
Catalog No.:TCH-C335L
Construction method:Lentivirus
Selection Marker:Puromycin
Parental:SK-OV-3
Species:Human
Morphology:Epithelioid
Growth properties:Adherent
Culture Medium:McCoy's 5A Medium supplemented with 10 % FBS, 2.5ug/mL Puromycin,100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G335L).
Passage:1:4-1:6
Doubling time:24-32 h
Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:6.
Freezing media:McCoy's 5A + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).