The SK-OV-3 cell line was derived in 1973 by G. Trempe and L. J. Old from the ascites fluid of a patient with ovarian tumor. SK-OV-3 cells are resistant to tumor necrosis factor and several cytotoxic drugs, including diphtheria toxin, cisplatin, and doxorubicin. SK-OV-3 cells are tumorigenic in nude mice and form moderately differentiated adenocarcinomas that are consistent with ovarian carcinoma in situ.

Cell Name: Human ovarian adenocarcinoma cell line

Cell Synonyms: SK-OV-3; SKOV-3; SK.OV.3; SKOV3; Skov3; SKO3

Catalogue No.: TCH-C335

Species: Human

Tissue Origin: Ovary

Disease Characteristics: Human Ovarian Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: McCoy's 5A + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G335

Passage Ratio: 1:4-1:6, with medium renewal every 2-3 days
Doubling Time: 24-32 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection