The SK-N-SH cell line was established by J.L. Bieder. It differs from the SK-N-MC cell line in that it has a longer doubling time and higher levels of dopamine-β-hydroxylase. SK-N-SH is used as a target cell line in cell-mediated cytotoxicity assays.

Cell Name: Human neuroblastoma cell line

Cell Synonyms: SK-N-SH; SK N SH; SKN-SH; SK-NSH; SKNSH; NSH

Catalogue No.: TCH-C334
Species: Human
Tissue Origin: Brain neuroblastoma, metastatic site: bone marrow
Disease Characteristics: —
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium:MEM + 10% FBS + 1% NEAA + 1% Glutamax + 1% P/S
Matching Medium Catalog Number:TCH-G334
Passage Ratio: 1:2-1:4, 2-3 times per week

Doubling Time: 44 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection