The SK-N-MC cell line is one of two neural tissue-derived cell lines established by Biedler·J·L (the other being the SK-N-SH cell line); it was isolated in September 1971. SK-N-MC cells exhibit moderate dopamine-β-hydroxylase activity and contain intracellular catecholamines that can be indicated by formaldehyde-induced fluorescence. Initially considered a neuroblastoma cell line, it was later confirmed to originate from an Askin tumor.

Cell Name: Human neuroepithelioma cell line

Cell Synonyms: SK-N-MC; SKNMC; SK-NM-C; SK-NMC

Catalogue No.: TCH-C333

Species: Human

Tissue Origin: Brain

Disease Characteristic: Metastatic focus of supraorbital region neuroepithelioma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: MEM + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G333

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection