The SJSA-1 cell line (previously known as OsA-CL) was established in 1982 from the primary tumor of a patient diagnosed with primary pleomorphic sarcoma of the femur.

Cell Name: Human osteosarcoma cell line (STR authenticated)

Cell Synonyms: SJSA-1; SJSA1; SJSA; OsA-CL; OSA-CL; OsACL; OSACL; Os-A; OSA; OsA

Catalogue No.: TCH-C327

Species: Human
Tissue Origin: Bone
Disease Characteristic: Osteosarcoma
Morphology: Epithelial-like
Growth Characteristics: Adherent growth
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G327
Passage Ratio: 1:3–1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma