This cell line originates from surgically resected tumor tissue of a Japanese patient. Electron microscopy reveals typical desmosomes between cells and abundant tonofilaments in the cytoplasm. Mycoplasma contamination was detected in 1975 and subsequently eliminated. The cells are integrated with the HPV16 genome, with 1–2 copies per cell.

Cell Name: Human cervical squamous cell carcinoma cell line (STR authenticated)

Cell Synonyms: SiHa; Siha; SIHA

Catalogue No.: TCH-C326

Species: Human
Tissue Origin: Cervix
Disease Characteristic: Cervical squamous cell carcinoma
Morphology: Epithelial-like
Growth Characteristics: Adherent growth
Culture Medium: DMEM-H + 10% FBS + 1% P/S
Matching Medium Code: TCH-G326
Passage Ratio: 1:3–1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma