This cell line is one of the many human tumor cell lines isolated and characterized by Fogh J and Trempe G. The cells were derived from the osteosarcoma tissue of an 11-year-old Caucasian female. The patient had undergone radiotherapy as well as treatment with various drugs, including methotrexate, doxorubicin, vincristine, cyclophosphamide, and aramycin-C. The cells are non-tumorigenic in immunosuppressed mice and express epidermal growth factor (EGF) receptors, as well as transforming growth factor β (type 1 and type 2) receptors.

Cell Name: Human osteosarcoma cell line (STR authenticated)

Cell Synonyms: Saos-2; SAOS-2; SAOS 2; Saos 2; Saos2; SaOs2; SAOS2; Sarcoma OSteogenic-2; SaOS; SAOS

Catalogue No.:TCH-C315

Species: Human

Tissue Origin: Bone

Disease Characteristic: Osteosarcoma

Morphology: Epithelial-like

Growth Characteristics: Adherent

Culture Medium: McCoy’s 5A + 15% FBS + 1% P/S

Matching Medium No.: TCH-G315

Passage Ratio: 1:3–1:6; change medium every 2–3 days

Doubling Time: 48–72 hours

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma