RPMI 8226 is a B lymphocyte that was isolated from the peripheral blood of a 61-year-old, male with plasmacytoma. The cell can be used in assay development and immunology research.

Cell Name: Human multiple myeloma cell line

Cell Synonyms: RPMI-8226; RPMI 8226; RPMI.8226; RPMI8226; RPMI no 8226; 8226; RPMI 8226/S; RPMI-8226S; RPMI8226/S; 8226/S; Roswell Park Memorial Institute 8226; GM02132; GM2132; GM 2132; Simpson

Catalogue No.: TCH-C312

Species: Human

Tissue Origin: Peripheral Blood

Disease Characteristic: Plasma Cell Myeloma

Morphology: Lymphoblast-like

Growth Properties: Suspension growth

Culture Medium: RPMI-1640 + 20% FBS + 1% P/S

Matching Medium Catalog Number: TCH-G312

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 48-72 h

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection

Key Points for Culturing RPMI-8226 Cells

1. Growth Pattern:
   The cells primarily grow as single suspended cells, but some may adhere to the flask (sometimes up to 50%).

2. Cell Size:
   The cell population is heterogeneous in size.

3. Passaging Method:

   • Recommended seeding density: 3–5 ×10⁵ viable cells/mL.

   • Maximum growth density should not exceed 2–3 ×10⁶ viable cells/mL.

   • This cell line can be maintained by adding fresh medium, centrifugation, or complete medium exchange.

   • Optimal passaging density: 5 ×10⁵ – 2 ×10⁶ viable cells/mL.

   • Medium change frequency: recommended 2–3 times per week.

4. Precautions:
   (1) Strictly control the passaging density; both too low and too high densities can easily lead to cell death.
   (2) When cell density is low, minimize handling and centrifugation to reduce cell damage.
   (3) Glutamine at 2 mM can be supplemented if the cell condition is poor.
   (4) Excessive cell debris may appear during culture; it can be removed by low-speed centrifugation (typically 800 rpm). (Note: Low-speed centrifugation may result in some cell loss, so this method is not suitable when cell density is very low.)
   (5) During cryopreservation and revival, ensure proper seeding density. After revival, maintain a stable culture environment, and generally observe cell status after 24 hours.