RKO is a poorly differentiated human colon cancer cell line. RKO cells contain wild-type p53 but lack the human thyroid hormone receptor nuclear receptor (h-TRβ1). The level of p53 protein in RKO cells is higher than that in RKO-E6 cells. The RKO cell line is the parental cell line for both RKO-E6 and RKO-AS45-1. This cell line is tumorigenic in nude mice and can form colonies in soft agar.

Cell Name: Human colon carcinoma cell line (STR authenticated)

Cell Synonyms: RKO

Catalogue No.: TCH-C308

Species: Human
Tissue Origin: Colon
Disease Characteristic: Colon Cancer
Morphology: Epithelial-like
Growth Properties: Adherent
Culture System: MEM + 10% FBS + 1% P/S
Matching Medium No.: TCH-G308
Passage Ratio: 1:3 to 1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma