The RD cell line is different from the TE 671 cell line, but it is also its parental cell.

Cell Name: Human rhabdomyosarcoma cell line

Cell Synonyms: RD; R D; RD-2; RD 2; 130T; 130-T; 130 T; TE-32; TE 32; TE32; TE 32.T; Te 32.T

Catalogue No.: TCH-C306
Species: Human
Tissue Origin: Muscle
Disease Characteristic: Rhabdomyosarcoma
Morphology: Spindle-shaped cells, multinucleated cells

Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G306

Passage Ratio: 1:4-1:6, with medium renewal every 2-3 days
Doubling Time: 24-35 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection