The Raji cell line, which exhibits a lymphoblast-like morphology, was derived from a Burkitt's lymphoma in the left maxilla of an 11-year-old Black boy. It is EBNA-positive.
Passage Ratio:4×105 cells/mL, with medium renewal every 2-3 days Doubling Time: 24-36 h Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201 Quality Control: Negative for bacteria, fungi, and mycoplasma detection