Cell Name: Human hepatocellular carcinoma cell line (STR profile confirmed)
Cell Synonyms: PLC/PRF/5; PLC-PRF-5; PLC PRF 5; PLC/PRF5; PLCPRF5; PLC-8024; PLC8024; PLC; Alexander cells; Alexander; Primary Liver Carcinoma/Poliomyelitis Research Foundation/5

Catalogue No.: TCH-C298

Species: Human

Tissue Origin: Liver
Disease Characteristic: Hepatocellular carcinoma

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: MEM + 10% FBS+1% P/S
Matching Medium Catalog Number: TCH-G298

Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 48-96 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection