Product Name：Luciferase Reporter PC-3 Cell Line

HyCyte cell line：HyCyte PC-3-Luc

Cat. code：TCH-C297L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：PC-3

Species：Human

Morphology：Epithelial-like

Growth properties：Adherent

Culture Medium：Ham's F-12K supplemented with 10 % FBS,8.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G297L).

Passage：1:3-1:6

Doubling time：72-96 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：Ham's F-12K + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).