PC-3 was derived from a bone metastasis of a 62-year-old Caucasian male with grade IV prostate adenocarcinoma; exhibits low levels of acid phosphatase activity and 5-alpha-testosterone reductase activity.

Cell Name: Human prostate adenocarcinoma cell line (STR-authenticated)

Cell Synonyms: PC-3; PC3; PC.3

Catalogue No.: TCH-C297

Species: Human
Tissue Origin: Prostate
Disease Characteristics: Prostate cancer
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: F12K + 10% FBS + 1% P/S
Matching Medium No.: TCH-G297
Passage Ratio: 1:3-1:6, with medium change every 2-3 days
Doubling Time: 72-96 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma