The NK-92 cell line is an IL-2-dependent natural killer (NK) cell line derived from the peripheral blood mononuclear cells of a 50-year-old Caucasian male with aggressive non-Hodgkin's lymphoma. The NK-92MI cell line is an IL-2-independent NK cell line derived from NK-92 through transfection. The parental NK-92 cells were transformed using the retroviral MFG-hIL-2 vector carrying human IL-2 cDNA via microsomal gene transfer. The transformation is likely stable due to the integration of the vector into the genomic DNA. This cell line exhibits cytotoxicity against many malignant cells; chromium release assays have shown that it can kill K562 and Daudi cells. The NK-92 cells are positive for the following surface markers: CD2, CD7, CD11a, CD28, CD45, and CD54; and negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34, and HLA-DR. Both the parental IL-2-dependent NK-92 cell line and another IL-2-independent variant derived from NK-92, known as NK-92CI, are available from ATCC. Both variants, NK-92MI and NK-92CI, contain, express, and synthesize hIL-2 cDNA. The NK-92MI cell line synthesizes higher levels of IL-2 compared to NK-92CI, whereas the parental cells do not produce or express IL-2.

Cell Name: Natural Killer-92 MI cell line

Cell Synonyms: NK-92MI; NK-92 MI; NK-92 mi; NK92-MI; NK92MI; NK-92 transfected with MFG-hIL2

Catalogue No.: TCH-C293
Species: Human
Tissue Origin: Peripheral Blood
Disease Characteristics: Malignant Non-Hodgkin's Lymphoma
Morphology: Lymphoblastoid
Growth Properties: Suspension

Culture Medium: MEMα + 12.5% FBS + 12.5% HS + 0.2 mM Inositol + 0.1 mM β-mercaptoethanol + 0.02mM Folic Acid + 1% P/S
Matching Medium Catalog Number: TCH-G293

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection