The NK-92 cell line is an IL-2-dependent natural killer (NK) cell line derived from the peripheral blood mononuclear cells of a 50-year-old Caucasian male with progressive non-Hodgkin's lymphoma. NK-92 cells exhibit cytotoxicity against a wide range of malignant cells; chromium release assays have shown that they can kill K562 and Daudi cells. Irradiated NK-92 cells (to prevent proliferation) can be effectively used for in vitro immunodepletion of leukemia cells in the bloodstream without compromising the function of normal blood cells. The NK-92 cell line is characterized by positive expression of surface markers including CD2, CD7, CD11a, CD28, CD45, and CD54, and negative expression of CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34, and HLA-DR.

Cell Name: Human natural killer cell line

Cell Synonyms: NK-92; NK92

Catalogue No.: TCH-C292
Species: Human
Tissue Origin: Lymphoid
Disease Characteristics: Malignant Non-Hodgkin's Lymphoma
Morphology: Lymphoblast-like
Growth Properties: Suspension

Culture Medium: MEMα + 12.5% FBS + 12.5% HS + 0.2 mM Inositol + 0.1 mM β-mercaptoethanol + 0.02 mM Folic Acid + 200 U/mL IL-2+ 1% P/S
Matching Medium Catalog Number: TCH-G292

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection