Product Name：Luciferase/GFP Dual-Reporter OVCAR-3 Cell Line

HyCyte cell line：HyCyte OVCAR-3-Luc-GFP

Catalog No.：TCH-C291LG

Construction method：PiggyBac

Selection Marker：Puromycin

Parental：OVCAR-3

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium: RPMI-1640 supplemented with 20 % FBS,0.5 ug/mL Puromycin, 0.01mg/ml Insulin,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G291LG).

Passage：1:2-1:4

Doubling time：36-64 h

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).