This cell line was derived from the lymph node of a 43-year-old Caucasian male with large cell lung carcinoma. There is no submicroscopic or biochemical evidence of mucin production or squamous differentiation. The cell line expresses p53 mRNA at readily detectable levels, significantly higher than those found in normal lung cells. At the same time, there are no overall structural DNA aberrations that could indicate the presence of point mutations or very small variations. It stains positively for keratin and vimentin fibers, but negatively for neurofilament triplet proteins.

Cell Name: Human large cell lung carcinoma cell line

Cell Synonyms:NCI-H661; H661; H-661; NCIH661

Catalogue No.: TCH-C287

Species: Human
Tissue Origin: Lung
Disease Characteristics: Large Cell Lung Carcinoma

Morphology: Lymphoblast-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS +1% P/S
Matching Medium Catalog Number: TCH-G287
Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection