The NCI-H358 cell line was isolated in 1981 from the tumor tissue of a patient prior to the initiation of chemotherapy. Ultrastructural studies have shown that the cytoplasm of NCI-H358 cells contains characteristic structures of Clara cells. These cells express the major pulmonary surfactant protein SP-A and its RNA, but do not express SP-B or SP-C. The colony-forming efficiency of NCI-H358 cells in soft agar is 0.83%.

Cell Name: Human bronchioloalveolar carcinoma cell line

Cell Synonyms: NCI-H358; H358; H-358; NCIH358

Catalogue No.: TCH-C284

Species: Human
Tissue Origin: Lung
Disease Characteristics: Lung Cancer

Morphology: Lymphoblast-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS +1% P/S
Matching Medium Catalog Number: TCH-G284
Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection