This cell line was derived from a lymph node metastasis of lung mucosal epithelial cell carcinoma. The cell line was isolated using a chemically synthesized medium and subsequently adapted to serum-containing culture conditions. During cultivation, the cells retain the ultrastructural characteristics of mucosal epithelial cells and exhibit multiple markers of squamous differentiation.

Cell Name: Human lung mucoepidermoid carcinoma cell line

Cell Synonyms: NCI-H292;H292; H-292; NCI-HUT-292; Hut292; NCIH292
Catalogue No.: TCH-C283
Species: Human
Tissue Origin: Lung
Disease Characteristics: —
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G283
Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 48 h

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection