This cell line was established in November 1990. The tissue donor is a male non-smoker.

Cell Name: Human mesothelioma cell line

Cell Synonyms: NCI-H2452 [H2452]; H2452; H-2452; NCIH2452

Catalogue No.: TCH-C282

Species: Human
Tissue Origin: Mesothelioma
Disease Characteristics: —

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G282

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days

Doubling Time: 48 h

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection