The NCI-H23 cell line was derived from the pretreatment tumor tissue of a 51-year-old African American male patient with non-small cell lung cancer. It expresses C-myc, L-myc, v-src, v-abl, v-erbB, c-raf 1, Ha-ras, Ki-ras, and N-ras RNAs. The NCI-H23 cells carry a K-ras codon 12 mutation (Gly12→?); there is also a mutation at codon 246 of the p53 gene (ATC→ATG). These cells express heterologous mRNAs for PDGF A and B chains, as well as TGFα, TGFβ, and EGFR. They are positive for keratins 5, 8, and 18, positive for vimentin, negative for neurofilament proteins, and negative for L-dopa dehydrogenase. It has been reported that NCI-H23 cells form colonies in soft agar with an efficiency of 9.7%.

Cell Name: Human non-small cell lung adenocarcinoma cell line

Cell Synonyms: NCI-H23; NCI.H23; NCI H23; H23; H-23; NCIH23

Catalogue No.: TCH-C280

Species: Human
Tissue Origin: Lung
Disease Characteristics: Lung Cancer

Morphology: Lymphoblast-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS +1% P/S
Matching Medium Catalog Number: TCH-G280
Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection