The NCI-H226 [H226] cell line was isolated and established in 1980.

Cell Name: Human non-small cell lung squamous cell carcinoma cell line

Cell Synonyms:NCI-H226

Catalogue No.: TCH-C279

Species: Human
Tissue Origin: Pleural effusion

Disease Characteristics: Squamous cell carcinoma of the lung

Morphology: Lymphoblast-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+1%Glutamax+1% Sodium Pyruvate +1% P/S
Matching Medium Catalog Number: TCH-G279
Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection