Product Name：Luciferase Reporter NCI-H2228 Cell Line

HyCyte cell line：HyCyte NCI-H2228-Luc

Catalog No.：TCH-C278L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：NCI-H2228

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：RPMI-1640 supplemented with 10 % FBS,2.0ug/mL Puromycin,   100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G278L).

Passage：1:2-1:4

Doubling time：7-14 days

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media：RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).