Cell Name: Human non-small cell lung adenocarcinoma cell line

Cell Synonyms:NCI-H2228; H2228; H-2228

Catalogue No.: TCH-C278

Species: Human
Tissue Origin: Lung
Disease Characteristics: Lung Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS +1% P/S
Matching Medium Catalog Number: TCH-G278
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time:   7–14 days (this cell line grows slowly)
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte^® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection