The NCI-H1395 cell line was established in April 1986, with the tissue donor being a smoker consuming 15 packs of cigarettes per year; the NCI-BL1395 [BL1395] cell line is a lymphoblastoid cell line derived from the same patient.

Cell Name: Human non-small cell lung adenocarcinoma cell line

Cell Synonyms:NCI-H1395; H1395;H-1395; NCIH1395

Catalogue No.: TCH-C270

Species: Human
Tissue Origin: Lung
Disease Characteristics: Adenocarcinoma

Morphology: Lymphoblast-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS +1% P/S
Matching Medium Catalog Number: TCH-G270
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection