This is a finite cell line. Excessive passaging may lead to changes in cell growth characteristics. It is recommended to complete the experiments within 3 passages after receipt.

Cell Name: Human fetal lung fibroblast cell line (STR authenticated)

Cell Synonyms: MRC-5; MRC5; MRC 5; MRCV; MRC-V;   Medical Research Council cell strain-5

Catalogue No.: TCH-C263

Species: Human
Tissue Source: Lung
Disease Characteristics: /
Morphology: Fibroblast-like
Growth Characteristics: Adherent growth
Culture System: DMEM-H + 10% FBS + 1% P/S
Matching Medium No.: TCH-G263
Passage Ratio: 1:2 – 1:3, change medium every 2–3 days

Doubling Time: 48–72 hours

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma