The MOLT-4 cell line was established from the cells of a relapsed patient who had received combination chemotherapy with multiple drugs. This patient carried a G→A mutation at codon 248 of the p53 gene, and p53 was not expressed. MOLT-4 cells do not produce immunoglobulins or Epstein-Barr virus (EBV). Both MOLT-4 and MOLT-3 cell lines were derived from the same patient.

Cell Name: Human acute lymphoblastic leukemia T cell line

Cell Synonyms: MOLT-4; Molt-4; MOLT 4; Molt 4; MOLT.4; MOLT4; Molt4; GM02219; GM02219C; GM2219C

Catalogue No.: TCH-C262
Species: Human
Tissue Origin: Blood
Disease Characteristic: Leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G262
Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 24-48 h

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma detection