This cell line was isolated and established from the osteosarcoma tissue of a 13-year-old Caucasian female. It exhibits a flat morphology, low saturation density, low colony-forming efficiency in soft agar, and sensitivity to compounds and viral infections.

Cell Name: Human osteosarcoma cell line

Cell Synonyms: HOS
Catalogue No.: TCH-C261
Species: Human
Tissue Origin: Bone
Disease Characteristic: Osteosarcoma
Morphology: Epithelial-like

Growth Properties: Adherent

Culture Medium: MEM + 10% FBS + 1% NEAA + 1% P/S
Matching Medium Catalog Number: TCH-G261

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection