Product Name：Luciferase/GFP Dual-Reporter MM.1S Cell Line

HyCyte cell line：HyCyte MM.1S-Luc-GFP

Catalog No.：TCH-C260LG

Construction method：PiggyBac

Selection Marker：Puromycin

Parental：MM.1S

Species：Human

Morphology：Lymphoblastic

Growth properties：Suspension

Culture Medium： RPMI-1640 supplemented with 10 % FBS,0.1ug/mL Puromycin,   2mM Glutamax, 1mM Sodium Pyruvate,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G260LG).

Passage：1:2-1:4

Doubling time：48-96 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media：RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).