Cell Name: Human highly metastatic hepatocellular carcinoma cell line (STR-authenticated)

Cell Synonyms: MHCC97H; MHCC 97-H; MHCC-97H

Catalogue No.: TCH-C258

Species: Human

Tissue Origin: Liver

Disease Characteristic: High metastatic potential hepatocellular carcinoma cells

Morphology: Epithelial-like

Growth Characteristics: Adherent growth

Culture System: DMEM-H + 10% FBS + 1% Glutamax + 1% Sodium Pyruvate + 1% P/S

Matching Medium No.: TCH-G258

Passage Ratio: 1:3–1:6, change medium every 2–3 days

Doubling Time: 48–72 hours

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma