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The MEG-01 cell line was derived from the bone marrow cells of a CML (chronic myeloid leukemia) patient during the megakaryoblastic transformation phase. The cytoplasm is positive for factor VIII and surface glycoproteins IIb/IIIa, as well as the periodic acid-Schiff (PAS) reaction; it is also positive for α-naphthyl acetate esterase and acid phosphatase. It is negative for myeloperoxidase, α-naphthyl butyrate esterase, chloroacetate AS-D naphthol esterase, and alkaline phosphatase. Staining with monoclonal antibodies BA-1 (anti-B cell, granulocyte), HPL-3 (anti-glycoprotein IIb/IIIa), and 20.3 (anti-monocyte, platelet) yields positive results, while other lymphoid and myeloid lineage antibodies yield negative results.
Cell Name: Human megakaryoblastic leukemia cell line
Cell Synonyms: MEG-01; Meg-01; MEG01; Meg01
Catalogue No.: TCH-C255
Species: Human
Tissue Origin: Blood
Disease Characteristic: Leukemia
Morphology: Lymphoblast-like
Growth Properties: Semi-adherent and semi-suspension
Culture Medium: RPMI-1640+ 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G255
Passage Ratio: 1:2-1:2, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection
Translation of MEG-01 Cell Culture Key Points:
Cell Characteristics: Black dot-like debris is always present during cultivation and requires no special treatment. Most cells exhibit irregular round shapes in suspension, while a small portion appear spindle-shaped and adherent.
Culture Method (Using T25 Flask as Example)
(1) MEG-01 is a semi-adherent/semi-suspension cell line. Adherent cells may account for ≤50% of the total, which is normal. Treat them as suspension cells during subculture: gently dislodge adherent cells by pipetting (use 0.25% trypsin if necessary).
(2) Maintain cell density at 3–10×10⁵ cells/mL. Initial seeding density should not be <3×10⁵ cells/mL; passage when density reaches ~10×10⁵ cells/mL. Early passages require cell counting, but experience-based subculture is acceptable later.
(3) Perform cell counting every 3 days. If density falls below 3×10⁵ cells/mL, transfer cells to smaller vessels (e.g., multi-well plates).
Subculture Methods (Choose Based on Density)
(1) Partial Medium Change: For densities <8×10⁵ cells/mL. Aspirate half of the supernatant, centrifuge at 1200 rpm (250g) for 3 min, resuspend in fresh medium, gently pipette 3–5 times, and return to the original flask.
(2) 1:2 Dilution Passage: For densities near 10×10⁵ cells/mL. Split cells equally into two new flasks, supplementing each with fresh medium to a total volume of 5 mL.
(3) Full Centrifugation/Passage: Perform weekly if cells are cultured >1 week. Avoid frequent centrifugation; recommended: 1200 rpm for 3 min.
MEG-01 Cell Culture Notes
(1) Slow proliferation and fragility: Minimize disturbance and maintain stable temperature/environment.
(2) Reduce pipetting: Gently mix 3–5 times during counting or subculture.
(3) Monitor daily post-passage. High density may cause cell death—strictly control density.
(4) Perform partial medium change or passage every 3 days; full centrifugation weekly.
(5) Recommended cryopreservation density: 3–5×10⁶ cells/mL.