The ME-180 originates from an irregularly shaped, non-keratinized invasive squamous cell carcinoma colony. In monolayer cultures, belt-like junctions can be observed between ME-180 cells, and cytoplasmic tonofilaments have also been noted. In 1970, mycoplasma contamination was detected and subsequently removed. TNF-α inhibits the growth of ME-180 cells; these cells contain human papillomavirus (HPV) DNA, showing greater homology with HPV-39 than with HPV-18.

Cell Name: Human cervical squamous cell carcinoma cell line

Cell Synonyms: ME-180; Me-180; ME 180; ME180

Catalogue No.: TCH-C254
Species: Human
Tissue Origin: Cervix
Disease Characteristic: Cervical Epidermoid Carcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: McCoy's 5A + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G254
Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection