$ 1000.00
Human breast adenocarcinoma cell line, cultured in L-15(MDA-MB-468)
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Cat.No.
TCH-C253
Size
1x10^6
Price
$1000.00


This cell line was isolated in 1977 by Cailleau R et al. from the pleural effusion of a 51-year-old African American woman with metastatic breast cancer. Although the donor tissue was heterozygous for the G6PD allele, this cell line consistently exhibits the G6PD A phenotype. There is a G→A mutation at codon 273 of the P53 gene, resulting in an Arg→His substitution. Each cell expresses 1×10⁶ EGF receptors.


Cell Name: Human breast ductal carcinoma cell line

Cell Synonyms: MDA-MB-468; MDA-MB 468; MDA-MB468; MDAMB468; MDA-468; MDA468; MB468; MD Anderson-Metastatic Breast-468

Catalogue No.: TCH-C253
Species: Human
Tissue Origin: Breast
Disease Characteristics: Breast Cancer
Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: L-15 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G253
Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 72-96 h
Culture Conditions: Atmosphere: Air , Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection


Notes:

  1. This cell line is recommended to be cultured in Leibovitz's L-15 medium. Leibovitz's L-15 medium must not be exposed to carbon dioxide, as CO₂ will cause cytotoxicity.

  2. If you do not have a CO₂-free incubator, DMEM medium can be used as a substitute for Leibovitz's L-15. When using DMEM, normal 5% CO₂ incubation is acceptable.

  3. The standard accompanying culture medium is configured for Leibovitz's L-15. If you require the DMEM formulation, please contact our sales team to place an order and note that you would like it changed to Human Breast Cancer Cell Line (DMEM-H medium)(TCH-C468).



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