In 1976, R. Cailleau et al. established the MDA-MB-453 cell line from the pleural effusion of a 48-year-old Caucasian female patient with metastatic tumors. Other metastatic sites included lymph nodes, brain, pleural effusion, and pericardial effusion. This cell line overexpresses the FGF receptor.

Cell Name: Human breast adenocarcinoma cell line (STR-verified)
Cell Synonyms: MDA-MB-453; MDA-MB 453; MDA MB 453; MDA-MB453; MDAMB453; MDA-453; MDA453; MD Anderson-Metastatic Breast-453

Catalogue No.: TCH-C252
Species: Human
Tissue Origin: Breast
Disease Characteristics: —
Morphology: Epithelial-like, mixed growth with both adherent and suspension cells present.

Growth Properties: Semi-adherent/semi-suspension
Culture Medium: L-15 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G252
Passage Ratio: 1:2-1:4, with medium change every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: Air; Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma.

Notes:

1. MDA-MB-453 cells exhibit mixed growth of adherent and suspension cells. Adherent cells display an epithelial-like morphology (cuboidal or polygonal), while suspension cells grow as single cells or clusters.

2. After resuscitation, most cells may appear in suspension and weakly adherent. Do not discard the suspension cells. During medium change or subculturing, collect and centrifuge the suspension cells, then reintroduce them into the culture flask for continued cultivation. After approximately 5 days of continuous culture, epithelial-like adherent cells will emerge.