Cell Name: Human breast adenocarcinoma cell line

Cell Synonyms: MDA-MB-436; MDA_MB_436; MDA MB 436; MDA-Mb-436; MDA-MB436; MDAMB436; MDA-436; MDA436; MB436; MD Anderson-Metastatic Breast-436

Catalogue No.: TCH-C251

Species: Human

Tissue Origin: Breast

Disease Characteristics: Breast Adenocarcinoma

Morphology: Pleomorphic

Growth Properties: Adherent

Culture Medium: L-15+20% FBS+0.01 mg/mL Insulin+ 1% P/S
Matching Medium Catalog Number: TCH-G251

Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: Air , Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection